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. 2014 Feb 13;9(2):e89012. doi: 10.1371/journal.pone.0089012

Figure 6. Effect of SCA platelets on endothelial gene expression.

Figure 6

Relative gene expression of (A) ICAM1, (B) NFKB1, (C) RELA, (D) CHUK and (E) IKKBETA in HUVEC after co-culture with PLTs. HUVEC (1×106 cells/well) were co-cultured in direct contact with CON (N = 10) or SCA PLTs (N≥24; 1×108 PLTs/well) (4 h, 37°C, 5% CO2); or pre-incubated with HUVEC with TNF-α (10 ng/ml; N≥11; 3 h, 37°C, 5% CO2). Relative gene expressions were determined by qPCR and expressed relative to ACTB and GAPDH expressions. *, P<0.05; **,P<0.01, compared to basal (N≥11). +++, P<0.001, compared to all groups.