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. Author manuscript; available in PMC: 2014 Feb 13.
Published in final edited form as: Nat Commun. 2013;4:3000. doi: 10.1038/ncomms4000

Figure 7. miR-205 is a flow-sensitive human homolog of murine miR-712.

Figure 7

(a) The highlighted region in yellow shows the seed sequence match between the murine miR-712 and murine and human miR-205 and those shown in blue indicate additional conserved sequences. (b) The putative targets of miR-712 and miR-205 obtained from TargetScan were compared. Venn diagram depicts the common gene targets of miR-205 and miR-712. (c) Expression of precursor- and mature miR-205 in iMAECs exposed to static, LS or OS for 24 h (n=4; data shown as means ± s.e.m; *, p<0.05 as determined by Student’s t-test). (d) Expression of precursor- and mature miR-205 in human aortic endothelial cells (HAECs) exposed to static, LS or OS for 24 h (n=4; data shown as means ± s.e.m; *, p<0.05 as determined by Student’s t-test). (e) Expression of mature-miR-205 was determined using the RNAs obtained from the endothelial-enriched (intimal region) and the leftover medial and adventitia region (M +A) of the LCA and RCA at 24 h and 48 h post-partial ligation, respectively. Expression of TIMP3 was determined by qPCR in (f) iMAECs and (g) HAECs transfected with increasing concentrations of pre-miR-205 or control-pre-miR compared to vehicle controls (mock) (n=3; data shown as means ± s.e.m; * *, p<0.05 as determined by Student’s t-test).