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. 2013 Sep 19;(79):50951. doi: 10.3791/50951
Preparation of Percoll solution to a final density of 1.07 g/ml (ρ = 1.07)
Tube No. 1 2 3 4
Undiluted Percoll (ρ = 1.130) (ml) 2.96 5.92 8.88 11.84
1.5 M NaCl (ml) 0.6 1.20 1.8 2.4
distilled H2O (ml) 2.44 4.88 7.32 9.76
Volume of final working dilution 6 ml 12 ml 18 ml 24 ml