| Preparation of Percoll solution to a final density of 1.07 g/ml (ρ = 1.07) | ||||
| Tube No. | 1 | 2 | 3 | 4 |
| Undiluted Percoll (ρ = 1.130) (ml) | 2.96 | 5.92 | 8.88 | 11.84 |
| 1.5 M NaCl (ml) | 0.6 | 1.20 | 1.8 | 2.4 |
| distilled H2O (ml) | 2.44 | 4.88 | 7.32 | 9.76 |
| Volume of final working dilution | 6 ml | 12 ml | 18 ml | 24 ml |