| Sample | Sample type | Antibody | Cell Number | Total volume |
| 1. unstained | control | 1 x 106 | 50 μl | |
| 2.scc*-Pacific Blue | control | CD45-Pacific Blue | 1 x 106 | 50 μl |
| 3. scc-APC | control | CD3-APC | 1 x 106 | 50 μl |
| 4.scc-Alexa 488 | control | CD8-Alexa 488 | 1 x 106 | 50 μl |
| 5. Cell sorting | analyte | CD45-Pacific Blue EpCAM-APC CDR2-Alexa 488 | 10 x 106 - 50 x 106 | 500 μl 10 x 106 cells/100 μl |
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