Figure 7.

Hepatocyte growth factor-dependent migration of myoblast in vivo requires glypican-1 expression. (A) Wild-type (WT) or C6 myoblasts (500 × 10³), prelabeled with the vital dialkylcarbocyanine dye, DiI (red fluorescence), and suspended in 30 μl of physiological serum with or without 10 ng of carrier-free hepatocyte growth factor (HGF) were transplanted into the left and right tibialis anterior (TA) muscles, respectively, of 3-month-old C57BL/10 mice under anesthesia. One week later, both TA muscles were processed for cryosectioning. Concentric circles with annuli 200 μm from each other were superimposed on selected muscle cross-section images. The cells were counted under an inverted microscope equipped for epifluorescence. (B) The number of the cells that migrated more than 200 μm were considered the total migrating cells, and the percentages of cells that migrated more than 600 μm were calculated. The migration of untreated WT myoblasts was corrected to 100%. Values are expressed as mean ± SD of two independent experiments. ***P < 0.001.