Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Feb 1;28(3):220–230. doi: 10.1101/gad.234591.113

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Grob et al.; Published by Cold Spring Harbor Laboratory Press

This article, published in Genes & Development, is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.

PMC Copyright notice

Figure 6. — Compartmentalized neonucleoli occupy distinct territories when integrated into endogenous nucleoli. (A) Combined 3D immuno-FISH performed on AMD-treated clone m1 reveals the presence of appropriately compartmentalized neonucleoli derived entirely from the neo-NOR. The top panels show UBF immunostained FC/DFC caps containing neo-NOR DNA (XEn probe; left panel) and lacking endogenous rDNA (IGS probe; right panel). The bottom panels show neonucleoli with a Nop52 immunostained GC associated with neo-NOR DNA (XEn probe; left panel) but lacking endogenous rDNA (IGS probe; right panel). Neonucleoli are indicated by arrowheads. In the enlarged insets, GCs, identified by low DAPI staining, are highlighted with a dashed line. (B, top panel) RNA-FISH performed on metacentric clone m1 identifies a distinct subnucleolar neo-NOR territory. Neo-NOR and endogenous transcripts are detected with mouse and human 5′ ETS probes, respectively. (Bottom panel) FISH performed on neo-NOR clone m1 indicates that neo-NORs and endogenous NORs are resolved into distinct nucleolar caps associated with the same GC upon AMD treatment. Neo-NORs and endogenous NORs were identified using XEn and IGS probes, respectively. GCs are identified by low DAPI staining. Images presented in the bottom panel correspond to single optical planes selected from deconvolved Z stacks.