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. 2014 Feb 1;28(3):220–230. doi: 10.1101/gad.234591.113

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© 2014 Grob et al.; Published by Cold Spring Harbor Laboratory Press

This article, published in Genes & Development, is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.

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Figure 7. — Formation of neonucleoli is UBF-dependent. (A) Combined 3D immuno-FISH performed on neo-NOR clone m1 transfected with control or UBF siRNA. Note that the loss of UBF is associated with the compaction of neo-NOR sequences (XEn probe). (B) Combined 3D immuno-FISH performed on neo-NOR clone m1 transfected with control or UBF siRNA reveals that neo-NOR sequences (XEn probe) devoid of associated UBF fail to recruit fibrillarin. Further images from these experiments are shown in Supplemental Figure S11.