Figure 5.

Sir3 and Sir4 enrichment in a strain lacking Sir2 catalytic activity revealed nucleation sites. 13xmyc-tagged Sir3 (top) and Sir4 (bottom) enrichment in a sir2N345A mutant shown at HML (left) and HMR (right). Salient features in the genome are shown below the X-axis, as above. Values are reported as sequence reads relative to the genome-wide median for the immunoprecipitate (IP; black) and the input (gray). For a reference, the green track shows the distribution of the Sir3 or Sir4 immunoprecipitate in chromatin from cells with wild-type Sir2, as in Figure 1.