Figure 7.
H3 was recovered in cross-linked samples at loci of site-specific transcriptional repression. Comparison of ChIP-seq of MNase-treated H3 immunoprecipitate (IP) from native chromatin (green) and chromatin cross-linked for 15 min (purple) at the GAL1–10 gene pair (A) and STE3 (B). Tick marks denote 1-kb intervals, and ORFs are shown below the X-axis. Reads were trimmed with nucleR to facilitate nucleosome mapping (Flores and Orozco 2011).