FIGURE 4.

Effect of SecA depletion on localization of DivIVA-GFP. (A,B) Localization of DivIVA-GFP in the secA depletion strain 4072 grown in the presence of 1 mM IPTG (A) or in the absence of IPTG (B). The corresponding phase contrast images are presented in the left panels. (C,D) Localization of untagged DivIVA after SecA depletion. Localization of DivIVA in the secA depletion strain BSN52 grown with 1 mM IPTG (C) or without the inducer (D). DivIVA localization was analyzed by immunofluorescence microscopy using the DivIVA antiserum 2 h after induction with 0.5% xylose. Note that longer induction times would be required for full complementation of the filamentous ΔdivIVA phenotype. Merged images are composed of red DAPI stained nucleoids and green DivIVA. Depletion of SecA and normal DivIVA expression was confirmed by Western blot analysis (E).