Table 1.
B. subtilis strains and plasmids used in this work.
| Strain/plasmid | Relevant characteristics | Source*/reference |
|---|---|---|
| Bacillus subtilis strains | ||
| 168 | trpC2 | lab collection |
| AZ1 | trpC2 secAT128A | Nakane et al. (1995) |
| IsecA | trpC2 secA′ erm Pspac-secA | Jongbloed et al. (2002) |
| NIG1121 | metB101 hisH101 | Sadaie and Kada (1985) |
| NIG1152 | metB101 hisH101 secA341 | Sadaie and Kada (1985) |
| 1803 | trpC2 ΩdivIVA::(PdivIVA-divIVA-gfp cat) | Thomaides et al. (2001) |
| 3308 | trpC2 ΩdivIVA::(PdivIVA-divIVA-gfp-his6 erm) | pQEDG1 → 168 |
| 4041 | trpC2 ΔdivIVA::tet | Oliva et al. (2010) |
| 4066 | trpC2 ΩamyE::(Pxyl-divIVA-gfp spc) | pDG9→168 |
| 4067 | trpC2 ΔdivIVA::tetΩamyE::(Pxyl-divIVA-gfp spc) | 4066→4041 |
| 4072 | trpC2 ΔdivIVA::tetΩamyE::(Pxyl-divIVA-gfp spc) secA′ erm Pspac-secA | IsecA→4067 |
| 4097 | trpC2 ΩamyE::(PdivIVA-gfp-his10 erm) | pQEG2→168 |
| BSN3 | trpC2 ΩamyE::(Pxyl-divIVA-gfpA206K spc) | pSH3→168 |
| BSN47 | trpC2 ΩamyE::(Pxyl-divIVA spc) secA′ erm Pspac-secA | pSH19→IsecA |
| BSN49 | trpC2 ΩamyE::(Pxyl-divIVA spc) | pSH19→168 |
| BSN50 | trpC2 ΩamyE::(PdivIVA-his6-divIVA spc) | pSH18→168 |
| BSN51 | trpC2 ΔdivIVA::tet ΩamyE::(Pxyl-divIVA spc) | 4041→BSN49 |
| BSN52 | trpC2 ΔdivIVA::tet ΩamyE::(Pxyl-divIVA spc) secA′ erm Pspac-secA | 4041→BSN47 |
| BSN61 | metB101 hisH101 ΩamyE::(Pxyl-divIVA-gfpA206K spc) | BSN3→NIG1121 |
| BSN62 | metB101 hisH101 secA341 ΩamyE::(Pxyl-divIVA-gfpA206K spc) | BSN3→NIG1152 |
| BSN66 | metB101 hisH101 ΔdivIVA::tet ΩamyE::(Pxyl-divIVA-gfpA206K spc) | 4041→BSN61 |
| BSN67 | metB101 hisH101 secA341 ΔdivIVA::tet ΩamyE::(Pxyl-divIVA-gfpA206K spc) | 4041→BSN62 |
| BSN73 | trpC2 ΩamyE::(PdivIVA-gfp-his6 erm) | pSH34→168 |
| BSN77 | trpC2 ΔdivIVA::tet ΩamyE::(Pxyl-divIVA-gfp spc) secY′ cat Pspac-secY | pDY6→4067 |
| BSN131 | trpC2 ΩsecA::(Pspac-secA2141-2536-his12 erm) | pSH68→168 |
| BSN158 | trpC2 secA′ erm Pspac-secA ΩaprE::(PdivIVA-divIVA-gfp spc) | pSH84→IsecA |
| BSN164 | trpC2 secA′ erm Pspac-secA ΩaprE::(PdivIVA-divIVA-gfp spc) ΩamyE::(PsecA-secA12 cat) | pSH98→BSN158 |
| BSN226 | trpC2 secAT128A ΩamyE::(Pxyl-divIVA-gfp spc) | pDG9→AZ1 |
| BSN228 | trpC2 secAT128A ΩamyE::(Pxyl-divIVA-gfp spc) ΔdivIVA::tet | 4041→BSN226 |
| Plasmids | ||
| pAPNC213 | bla aprE5′ spc lacI aprE3^′ | Morimoto et al. (2002) |
| pDY6 | bla Pspac-secY lacI cat | Breitling et al. (1994) |
| pMUTinHis | bla erm Pspac-his12 lacI | Ishikawa et al. (2006) |
| pX | bla amyE5′ xylR cat amyE3^′ | Kim et al. (1996) |
| pDG7 | bla amyE3′ spc Pxyl-PdivIVA-divIVA-gfp amyE5^′ | Lenarcic et al. (2009) |
| pDG9 | bla amyE3′ spc Pxyl-divIVA-gfp amyE5^′ | Oliva et al. (2010) |
| pDG24 | bla amyE3′ spc Pxyl-PdivIVA-his6-divIVA-gfp amyE5^′ | this work |
| pQEDG1 | bla divIVA-gfp-his6 erm | Lenarcic et al. (2009) |
| pQEG2 | bla PdivIVA-gfp-his10 ′amyE^′ erm | Lenarcic et al. (2009) |
| pSH3 | bla amyE3′ spc Pxyl-divIVA-gfpA206K amyE5^′ | Oliva et al. (2010) |
| pSH18 | bla amyE3′ spc Pxyl-PdivIVA-his6-divIVA amyE5^′ | this work |
| pSH19 | bla amyE3′ spc Pxyl-divIVA amyE5^′ | this work |
| pSH34 | bla PdivIVA-gfp-his6 ′amyE^′ erm | this work |
| pSH68 | bla erm Pspac-secA2141-2536-his12 lacI | this work |
| pSH83 | bla amyE5′ PsecA-secA cat amyE3^′ | this work |
| pSH84 | bla aprE5′ spc lacI PdivIVA-divIVA-gfp aprE3^′ | this work |
| pSH98 | bla amyE5′ PsecA-secA12 cat amyE3′ | this work |
*The arrow (→) indicates a transformation where the donor and the recipient are given on the left and the right side of the arrow, respectively.