Figure 2.

Levels of relevant RNA-binding proteins. Protein extracts were prepared from paraspinal muscle tissues of mice and examined by western blots. Each lane represents a different mouse with the indicated genotype and induction condition. CELF1 was not detected in Celf1^−/− mice. Western blot for CELF2, MBNL1 showed no change due to absence of CELF1 or presence of RNA toxicity. Western blot for eGFP confirmed transgene induction. GAPDH was used as loading control (see Supplementary Material, Fig. S4 for graphical representation of quantifications).