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. 2013 Sep 2;23(2):293–302. doi: 10.1093/hmg/ddt419

Figure 6.

Figure 6.

MEF2A and C/EBPβ protein levels and expression of MuRF1 mRNA are affected by the absence of CELF1 in RNA toxicity. (A) Western blot for MEF2A. Numbers indicate relative intensity of bands (n = 4). MEF2A levels are not significantly affected by CELF1 deficiency in uninduced mice (P > 0.2, t-test). MEF2A increases significantly in Celf1+/+/5-313 with RNA toxicity (*P < 0.05, t-test). This is not seen in CELF1 deficient mice with RNA toxicity. (B) C/EBPβ LAP and (C) LIP isoforms were measured from western blots and relative protein expression are graphically represented. Both LAP and LIP levels were increased by RNA toxicity in induced 5-313 mice but not in Celf1−/−/5-313 mice (n = 5 per group). (D) Relative MuRF1 mRNA expressions were measured by qRT–PCR. Celf1+/+/5-313(+dox) mice (n = 6) have 5-fold more MuRF1 mRNA than uninduced mice (n = 7) and these levels are reduced to 1.6-fold in Celf1−/−/5-313(+dox) (n = 5). Significant differences were calculated and indicated as described in Figure 3. *P < 0.05, **P < 0.01 and ***P < 0.005 (t-test).