Fig. 1.

The E-cadherin–biotin-ligase fusion protein and biotinylated proteins colocalize with endogenous E-cadherin. (A) Myc-tagged EcadBL (Myc, middle) colocalized with endogenous E-cadherin (left and right, merge) detected using a canine-specific E-cadherin antibody that does not recognize the human EcadBL fusion protein. (B) In the presence (bottom panels) but not the absence (top panels) of exogenous biotin, fluorescent streptavidin signal (middle and right panels) colocalizes with the EcadBL fusion protein (Myc, left and right panels). Scale bars: 20 µm.