Table 2. Bemisia tabaci transcriptome resources used in this study.
B. tabaci B50 | B. tabaci Q51 | B. tabaci B49 | Cafa | Cuf | Tof | Cof | Cam | Cum | Tom | Com | Pooled | |
---|---|---|---|---|---|---|---|---|---|---|---|---|
Length distribution (bp) | ||||||||||||
0.1–0.5 k | 151,375 | 151,360 | 40,254 | 30,416 | 31,089 | 32,175 | 31,152 | 44,478 | 23,187 | 41,068 | 25,051 | 55,584 |
0.5–1.0 k | 22,546 | 12,949 | 13,007 | 11,259 | 12,885 | 12,599 | 11,234 | 15,864 | 6,934 | 13,808 | 6,127 | 17,091 |
1.0 k–2.0 k | 4,046 | 3,929 | 4,070 | 7,622 | 8,935 | 7,905 | 6,060 | 9,829 | 3,204 | 8,390 | 2,195 | 10,992 |
>2.0 k | 702 | 662 | 410 | 6,443 | 7,144 | 4,684 | 2,967 | 6,894 | 890 | 5,384 | 682 | 10,281 |
Total | 178,669 | 168,900 | 57,741 | 55,740 | 60,053 | 57,363 | 51,413 | 77,065 | 34,215 | 68,650 | 34,055 | 93,948 |
Sequencing statistics (bp) | ||||||||||||
Total (Mb) | 62.9 | 42.9 | 26.4 | 48.4 | 53.5 | 43.0 | 33.8 | 62.0 | 17.8 | 49.8 | 15.9 | 79.7 |
Mean | 368 | 266 | 478 | 911 | 934 | 786 | 689 | 843 | 543 | 761 | 490 | 889 |
Median | 327 | 172 | 355 | 444 | 478 | 435 | 403 | 417 | 331 | 388 | 335 | 392 |
Min | 102 | 100 | 200 | 201 | 201 | 201 | 201 | 201 | 201 | 201 | 201 | 201 |
Max | 5,872 | 7,297 | 5,936 | 16,744 | 19,356 | 16,173 | 17,748 | 24,983 | 12,022 | 19,970 | 9,551 | 27,456 |
N50 | 423 | 331 | 552 | 1,757 | 1,710 | 1,294 | 1,023 | 1,503 | 723 | 1,320 | 561 | 1,853 |
Comparative analysis | ||||||||||||
Platform | 454 | Illumina | Illumina | Illumina | Illumina | Illumina | Illumina | Illumina | Illumina | Illumina | Illumina | Illumina |
Assembly | gsAssembler | SOAPdenovo | SOAPdenovo | Trinity | Trinity | Trinity | Trinity | Trinity | Trinity | Trinity | Trinity | Trinity |
Hitb | 72.5% | 77.7% | 93.7% |
“a”: Ca, Cu, Co, and Tom are abbreviation for the respective host plants including Cabbage, Cucumber, Cotton and Tomato, while m and f denote males and females.
“b”: Blastn against the pooled transcriptome dataset using an e-value cutoff of 1e-5.