TABLE 2.
Plasmids used in this study
| Plasmid | Relevant marker | Source |
|---|---|---|
| pAZ7 | GST-DRG1 | (9) |
| pAZ8 | GST-drg1-1 | (9) |
| pDRG1-EQ1 | GST-drg1E346Q | (3) |
| pDRG1-EQ2 | GST-drg1E617Q | (3) |
| pDRG1-KA1 | GST-drg1K292A | This study |
| pDRG1-KA2 | GST-drg1K563A | This study |
| pRS315 | CEN, 2μ, LEU2 | (32) |
| pRS315-DRG1 | DRG1 | (3) |
| pRS315-drg1-1 | drg1-1 | This study |
| pRS315-drg1-3 | drg1-3 | This study |
| pRS315-drg1-4 | drg1-4 | This study |
| pRS315-drg1-11 | drg1-11 | This study |
| pGEX6P-1 | ori ampR lacIq PreScission Cleavage site GST tag | GE Healthcare |
| pGEX-Rlp24C | GST-RLP24C | (3) |
| pET32a | ori ampR lacIq Trx tag-S tag HIS6 tag | Novagen |
| pET32-Rlp24C | HIS6-RLP24C (441–599 bp) | (3) |
| pET28 | ori ampR lacIq HIS6 tag | Novagen |
| pET28-N172 | HIS6-NUP116 (1–516 bp) | (3) |