FIGURE 1.
GFP-Dcp1a expression inhibits poliovirus protein expression. A, A549 cells were transfected with 1 μg/1.5 × 105 cells of pcDNA4-GFP-Dcp1a and stained with an antibody against RCK/p54 (first panel). A549 cells were transfected with 1 μg/1.5 × 105 cells of pcDNA4-GFP (second panel), peGFP-Dcp1a (third panel), or peGFP-Dcp1a-ΔCTD (fourth panel) and infected with poliovirus (PV) (multiplicity of infection of 10) for 5 h. Cells were stained with an antibody against poliovirus 3Cpro (red). The highlighted area of the larger merged color image is separated into its constituent colors in the bottom panels. B, quantification of poliovirus titer resulting from infection of A549 cells expressing GFP or GFP-Dcp1a. Error bars indicate mean ± S.D. **, p < 0.0001. PFU, plaque-forming units.