Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Dec 18;289(7):3978–3990. doi: 10.1074/jbc.M113.529610

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 3. — Comparison of the digestion of a synthetic peptide substrate analyzed by MALDI-TOF MS and HPLC. A, peptide 7 was digested with Arg-528/Asp-575 at the indicated times in parallel experiments, and the digestion mixtures were analyzed by MALDI-TOF and HPLC, respectively. Conventions are as in Fig. 2. The left graph shows the combined digestion of peptide species longer than the natural ligand, excluding peptide 7 itself. Digestion was calculated as 100% − the combined yield of the corresponding species. The middle and right graphs show the % yield of the natural ligand and of shorter peptide(s), respectively. B, same substrate was digested with Lys-528/Asn-575 and analyzed as in A. The data in each panel are from a single experiment, but it was carried out separately for samples to be analyzed by either MS or HPLC.