TABLE 3.

HLA-B27 epitope destruction by ERAP1 variants

The data represent the maximal % yield of peptide species shorter than the natural HLA-B27 ligands (sequences highlighted in boldface) by the indicated ERAP1 variant. For each variant, the minimal and maximal (Max)values observed with the peptides tested are highlighted in boldface. Peptides 9 (RRYQKSTEL) and 10 (RRYQKSTELLIR) are not included. The data are means ± S.D. of at least three experiments.

Peptide		Arg-528/Asp-575, % Max	Lys-528/Asp-575, % Max	Arg-528/Asn-575, % Max	Lys-528/Asn-575, % Max
1	GRHHEAS.IRLPSQYNF	6.9 ± 0.4	9.1 ± 0.9	14.4 ± 1.1	78.1 ± 9.2
2	LGVFRKF.SRFPEALRL	0.3 ± 0.1	5.6 ± 1.2	67.5 ± 6.9	82.6 ± 6.7
3	NLKARNS.FRYNGLIHR	23.1 ± 2.7	25.7 ± 0.8	46.4 ± 1.8	81.7 ± 1.7
4	IMYKKRT.KRLVVFDAR	21.8 ± 2.5	27.5 ± 0.1	22.4 ± 3.0	66.6 ± 2.7
5	DVVYAL.KRQGRTLYGF	10.5 ± 0.7	3.6 ± 0.5	22.8 ± 2.1	99.9 ± 0.5
6	LYSESL.ARYGKSPYLY	58.5 ± 2.6	71.5 ± 2.3	78.4 ± 4.1	76.6 ± 2.6
7	LREI.RRYQKSTEL	14.1 ± 0.3	17 ± 3.5	23 ± 2.2	44.5 ± 2.6
8	LREI.RRYQKSTELLIR	44.5 ± 3.1	54.2 ± 2.1	62.2 ± 0.8	76.8 ± 1.3
	Mean	22.5	26.8	42.1	75.9