FIGURE 4.

Effects of siRNAs against PERK, ATF6, IRE1, and GRP78 on CD36 expression in ox-LDL-incubated RAW264.7 cells. Cells were transfected with siRNA directed against PERK (A), ATF6 (B), IRE1 (C), and GRP78 (D), followed by treatment with 50 mg/liter ox-LDL for 12 h. Expression of CD36 protein and mRNA were evaluated by Western blot and quantitative real-time PCR, respectively. Expression of the target gene of each siRNA, PERK (E), ATF6 (F), IRE1 (G), and GRP78 (H), was also quantified by real-time PCR. Data are expressed as mean ± S.E. of at least three independent experiments. *, p < 0.05, **, p < 0.01 versus control group transfected with con-siRNA; #, p < 0.05, ##, p < 0.01 versus ox-LDL group transfected with con-siRNA.