FIGURE 6.

CD36 silencing inhibits ox-LDL-induced ER stress response in RAW264.7 cells. Cells were transfected with a siRNA against CD36 or a negative control siRNA, followed by treatment with 50 mg/liter ox-LDL for 12 h. A, ER stress markers were evaluated by Western blot and normalized to β-actin. B, immunofluorescence experiments show ATF6 visualized by Cy3 labeling (red) and nuclei stained with DAPI (blue). Representative fluorescent images are shown. Scale bar, 20 μm. C, the protein level of ATF6 in nuclear extracts was analyzed by Western blot and normalized to histone level. D, the silencing of CD36 was validated by quantitative real-time PCR. Data are expressed as mean ± S.E. of at least three independent experiments. *, p < 0.05, **, p < 0.01 versus control group transfected with con-siRNA; #, p < 0.05 versus ox-LDL group transfected with con-siRNA.