Peripheral administration of recombinant CTRP4.
A, Western blot analysis of sera and cerebrospinal fluid from mice injected intraperitoneal with recombinant FLAG epitope-tagged CTRP4 (0.5 μg/g body weight). B, food intake at 1 and 24 h post-intraperitoneal injection of vehicle (n = 10) and recombinant CTRP4 (2 μg/g body weight, n = 4). C, body weight of mice pre- and post-intraperitoneal injection with vehicle (n = 10) and recombinant CTRP4 (n = 4). D, blood glucose of mice pre- and post-intraperitoneal injection of vehicle (n = 10) and recombinant CTRP4 (n = 4).