FIGURE 7.

Refeeding after fasting induces the expression of Ctrp4 in the hypothalamus. A–C, C57BL/6 male mice (∼8 weeks old, n = 7–8 mice/group) were fasted overnight or fasted overnight followed by 3 h of refeeding. A, different brain regions were harvested, and Ctrp4 expression was quantified by real-time PCR. B, CTRP4 expression was quantified by Western blotting. C, peripheral tissues were also harvested, and Ctrp4 expression was quantified by real-time PCR. D, Western blot quantification of serum CTRP4 levels in mice fasted overnight or fasted overnight followed by 3h of refeeding (n = 7 mice/group). PCR results were normalized to 18 S rRNA. Western blot analysis results were normalized to tubulin. Each lane represents a sample from a different mouse. **, p < 0.01 compared with the fasted group; NS, not significant.