FIGURE 2.
Proteolytic treatment diminishes TSP-1 binding activity of S. aureus. Binding of different concentrations of FITC-labeled hTSP-1 (50 and 100 μg/ml) to 2 × 108 S. aureus SA113. Bacteria were pretreated with either Pronase E (ProE, 1 mg/ml) to digest potential proteinaceous binding partners on the bacterial surface or with sodium periodate (NalO4, 0.05 mg/ml) to oxidize potential carbohydrate binding partners. Binding of FITC-labeled human TSP-1 was analyzed (n = 3) by flow cytometry in comparison with untreated bacteria (w/o). The values are represented as the geometrical mean fluorescence intensity multiplied with percent gated events (GMFI x % gated events). **, p < 0.01; ***, p < 0.001; ns, not significant.