FIGURE 3.

Increased mRNA and decreased protein expression of SRSF1 occurs independently of the proximal TCR signaling. T cells were activated with PMA and ionomycin for 0, 3, 6, and 24 h. A, cells were collected, and total RNA was isolated and reverse-transcribed. SRSF1 expression was measured by quantitative PCR and normalized to the cyclophilin A housekeeping gene. Graph shows average values from n = 3 donors, and error bars represent S.D. Stim., stimulated. B, total protein was extracted from T cells and immunoblotted for SRSF1 and β-actin. Representative blots are shown. Iono., ionomycin. C, densitometric quantitation of SRSF1 from immunoblots was performed and normalized to β-actin. Graph shows average values from n = 3 donors, and error bars represent S.D.