SIRT4 represses glutamine anaplerosis in human Burkitt lymphoma cells.
A and B, SIRT4 protein in whole cell lysates from Ramos (A) and Raji (B) cells treated with or without Dox (1 μg/ml) for 12 h was detected by immunoblotting. β-Actin served as a loading control. C and D, glutamine uptake in pEXP7, SIRT4, and SIRT4H161Y Ramos (C) and Raji (D) cells incubated with or without doxycycline for the indicated times (n = 3). E and F, ammonia production in pEXP7, SIRT4, and SIRT4H161Y Ramos (E) and Raji (F) cells incubated with or without doxycycline for the indicated times (n = 3). Data are mean ± S.E. *, p < 0.05 and **, p < 0.01.