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Effect of rapamycin on the expression of pyruvate dehydrogenase E1α and PYGB proteins in the heart. A, representative immunoblots of pyruvate dehydrogenase E1α, PYGB, and GAPDH in hearts of C57 and db/db mice following 28 days of RAPA treatment. B and C, densitometric analysis of the ratios of pyruvate dehydrogenase E1α to GAPDH (B) and PYGB to GAPDH (C).
