TABLE 1.
Protein name | 2D-DIGE spot | Molecular mass | (db/db)/wild-type C57 ratio | (db/dbRAPA)/(db/db) ratio | RAPA effect |
---|---|---|---|---|---|
kDa | |||||
Antioxidant proteins | |||||
Peroxisomal membrane protein 20 (peroxiredoxin 5) | 89 | 17.0 | 6.48↑ | 1.71↑ | Induced |
Ferritin heavy chain 1 | 86 | 21.1 | 1.59↑ | 1.57↑ | Induced |
Cytoskeletal/contractile proteins | |||||
Myosin, light polypeptide 2 | 83 | 18.9 | 2.06↑ | 1.98↓ | Reversed |
Myosin, heavy polypeptide 6α | 1 | 223.4 | 1.36↓ | 1.81↑ | Reversed |
Nebulette | 13 | 101.1 | 1.09↑ | 1.78↑ | Induced |
Myosin-binding protein C | 7 | 140.5 | 1.62↓ | 1.50↑ | Reversed |
Glucose metabolism proteins | |||||
Pgm2 | 33 | 63.4 | 1.40↓ | 2.24↑ | Induced |
Pyruvate dehydrogenase E1α | 59 | 43.2 | 1.02↑ | 1.71↓ | Reduced |
PYGB | 11,12 | 67.0 | 1.01, 1.03↓ | 1.7, 2.7↑ | Induced |
Proteins with other functions | |||||
α-Fetoprotein | 25 | 47.2 | 1.32↓ | 3.09↑ | Induced |
Fibrinogen-γ | 41,42 | 49.3 | 1.84↑, 1.11↓ | 2.22, 2.08↑ | Induced |
Fibrinogen-α | 36 | 63 | 1.49↓ | 2.38↑ | Induced |
Fibrinogen-β | 39,40 | 54.7 | 1.32↓, 1.3↑ | 1.86, 2.35↑ | Induced |
Carbonic anhydrase I | 75 | 28.3 | 1.38↑ | 1.96↑ | Induced |
Carbonic anhydrase II | 72 | 29 | 1.03↑ | 2.2↑ | Induced |
Hemoglobin-β | 94 | 15.6 | 1.79↑ | 1.99↑ | Induced |
Heat shock protein β-7 | 87 | 18.6 | 1.34↑ | 1.92↓ | Reduced |
Serine (or cysteine) proteinase inhibitor | 26 | 46.6 | 1.81↓ | 1.9↓ | Reduced |
Transferrin | 19 | 76.6 | 1.43↓ | 1.55↑ | Induced |
Pantothenate kinase 4 | 18 | 86.3 | 1.02↓ | 1.52↑ | Induced |
Prolyl 4-hydroxylase β | 27 | 58.9 | 1.85↓ | 1.5↑ | Induced |