FIGURE 2.
Kinetics of protein expression in WT and ΔmazF strains. Protein expression was monitored by GFP production using flow cytometry. WT (filled curves) and BWΔmazF (empty curves) strains carrying both the plasmid-borne degradable GFP as well as the E24A MazF mutant were induced at an A600 nm of 0.2 with 0.2% arabinose. GFP expression as a function of time after induction was monitored at regular intervals using flow cytometry. Substantial GFP expression is seen in both strains even after several hours of E24A MazF expression, although there was lower expression in the WT strain.