FIGURE 7.

The myosin II ATPase inhibitor, blebbistatin, does not impact complement-induced activation and relocalization of GEF-H1. GEC were stimulated with complement in the presence or absence of blebbistatin (20 μm) for 30 min. A, complement-induced activation of GEF-H1 assessed by pull-down was not affected by blebbistatin. *, p < 0.01; **, p < 0.05 versus HIS, n = 3. B, complement-induced perinuclear relocalization of GEF-H1 assessed as in Fig. 6 was not altered by blebbistatin. **, p < 0.01 versus HIS, n > 40 cells from three experiments. C, complement tended to decrease cell size, but the difference was not significant. Blebbistatin increased cell size significantly. *, p < 0.05 versus no blebbistatin, n > 40 cells from three experiments. (See “Experimental Procedures” for measurement of cell size.) Error bars, S.E.