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. 2013 Dec 20;289(7):4233–4243. doi: 10.1074/jbc.M113.530055

FIGURE 5.

FIGURE 5.

A cryoprotectant at the extracellular monolayer stabilizes the PM open state. Single channel steady-state current recordings (left) and their corresponding normalized all point histograms (right) of the PM in asymmetric lipid bilayers (A and B) with a lipid anchored cryoprotectant (A) DOPE-PEG350 or (B) DPhPE-containing monolayers colocalized with the filter gate and the DOPA-containing monolayer with the bundle gate. The distinct channel parameters are: γ = 55 ± 5 pS, Po = 0.61 ± 0.03, τopen = 4.1 ± 0.4 ms and burst length = 16 ± 2 ms for A and γ = 62 ± 3 pS, Po = 0.04 ± 0.01, τopen = 1.2 ± 0.2 ms and burst length = 5 ± 1 ms for B. Single channel steady-state current recordings (left) and their corresponding normalized all point histograms (right) of the PM in DOPA-containing symmetric lipid bilayers bathed in 0.5 m KCl on the PM bundle side and in 0.5 m KCl, 1% ethylene glycol (C) or 0.5 m KCl, 0.2 m mannitol (D) on the filter gate.