FIGURE 6.

The lipid-modulated stabilization of the PM open state occurs at physiological ionic strength. Single channel steady-state current recordings (left) and their corresponding normalized all point histograms (right) of the PM in symmetric DPhPC bilayers supplemented with (A) DOPA and (B) DPhPG, and (C) in asymmetric bilayers doped with DOPA on the extracellular leaflet and lysyl-DOPG in the intracellular facing monolayer. All the recordings were acquired in 0.15 m KCl, 10 mm HEPES, pH 7.4, at V = 50 mV. Currents were further filtered to 300 Hz for analysis and presentation.