FIGURE 4.

Anti-EDA antibodies EctoD2 and EctoD3 recognize epitopes overlapping the EDA receptor-binding site. A, Fc-EDA1 WT or containing the indicated point mutations, or Fc-BAFF as a control, were coated onto ELISA plates and revealed with the indicated antibodies at the indicated concentration. B, space filling representation of EDA1 structure (from Protein Data Bank coordinate file 1RJ7), showing one of the EDA1 monomers on the left (mono 1) and one on the right (mono 2). Positions of point mutants used in Fc-EDA1 of A are shown in black. The predicted binding site of EDAR is shown as a shade of gray (receptor). In this representation, the membrane of the receptor-bearing cell would be at the bottom, and the collagen domain of EDA1 would be at the top.