FIGURE 6.

Receptor binding specificity of human and chicken EDA1 and EDA2. Receptors (human or chicken EDAR, XEDAR, or TROY) fused to the GPI anchor of TRAILR3 were expressed in 293T cells together with an EGFP tracer (x axis). Cells were stained with cell supernatants containing human or chicken Fc-EDA1 or Fc-EDA2. Expression of GPI-anchored receptors was monitored with a monoclonal antibody (mAb572) recognizing the C-terminal portion of TRAILR3 present in all receptors. Both scattergram axes show fluorescence intensity on a logarithmic scale (10⁰–10⁴).