FIGURE 8.

Anti-EDA monoclonal antibody EctoD2 blocks the therapeutic activity of Fc-EDA in vivo. Wild type or Eda-deficient Tabby mice were analyzed at weaning (3 weeks old) for the presence or absence of hair on the tail and functional sweat glands in footpads using a starch-iodine sweat assay. Eda-deficient mice were either left untreated or treated on the day of birth with Fc-EDA1 at 2.5 mg/kg that had been preincubated with a 4-fold mass excess of either EctoD1 (n = 3) or EctoD2 (n = 4) monoclonal antibodies, as indicated. All mice in a treatment group displayed identical phenotypes.