TABLE 1.
Plasmids used in this study
Signal, MAIIYLILLFTAVRG; Ig signal, METDTLLLWVLLLWVPGSTG; FLAG, DYKDDDDK; linker, RSPQPQPKPQPKPEPEGS; PreSci, LEVLFQGP; aa, amino acids.
| Plasmid | Designation | Protein encoded | Vector | Figure(s) |
|---|---|---|---|---|
| ps515 | EGFP | Enhanced green fluorescent protein | PCR3 | 5A and 6 |
| ps548 | FLAG-EDA1 | Signal-FLAG-GPGQVQLQVD-mEDA1 (aa 245–391) | PCR3 | 5B |
| ps930 | hEDAR-Fc | hEDAR (aa 1–183)-VD-hIgG1 (aa 245–470) | PCR3 | 5B |
| ps1196 | Fc-hBAFF | Signal-LD-hIgG1 (aa 245–470)-linker-PreSci-GSLQ-hBAFF (aa 137–285) | PCR3 | 4 |
| ps1235 | Fc-EDA2 | Signal-LD-hIgG1 (aa 245–470)-linker-LQVD-hEDA2 (aa 245–389) | PCR3 | 5A and 6 |
| ps1236 | Fc-EDA1 | Signal-LD-hIgG1 (aa 245–470)-linker-LQVD-hEDA1 (aa 245–391) | PCR3 | 4 |
| ps1431 | hEDAR-GPI | hEDAR (aa 1–183)-VD-hTRAILR3 (aa 157–259) | PCR3 | 5A and 6 |
| ps1432 | hXEDAR-GPI | Ig signal-DVT-hXEDAR (aa 1–134)-VD-hTRAILR3 (aa 157–259) | PCR3 | 5A and 6 |
| ps1434 | hTROY-GPI | hTROY (aa 1–168)-VD-hTRAILR3 (aa 157–259) | PCR3 | 6 |
| ps1661 | Fc-PS-EDA1 | Signal-LD-hIgG1 (aa 245–470)-linker-PreSci-GSLQVD-EDA1 (aa 245–391) | PCR3 | 5A and 6 |
| ps1938 | Fc-EDA1 | Signal-hIgG1 (aa 245–470)-hEDA1 (aa 238–391) | PCR3 | 2, 3, 5C, and 8 |
| ps2199 | hEDAR:Fas | hEDAR (aa 1–183)-VD-hFas (aa 169–335) | pMSCV | 5C |
| ps2290 | chEDAR-GPI | chEDAR (aa 1–183)-VD-hTRAILR3 (aa 157–259) | PCR3 | 5A and 6 |
| ps2303 | Fc-chEDA1 | Signal-LD-hIgG1 (aa 245–470)-linker-LQNSG-chEDA1 (aa 207–353) | PCR3 | 5A and 6 |
| ps2323 | chTROY-GPI | chTROY (aa 1–167)-VD-hTRAILR3 (aa 157–259) | PCR3 | 6 |
| ps2335 | Fc-chEDA2 | Signal-LD-hIgG1 (aa 245–470)-linker-LQ-chEDA1 (aa 207–351) | PCR3 | 5A and 6 |
| ps2340 | chXEDAR-GPI | Signal-LE-chXEDAR (aa 2–137)-GSVD-hTRAILR3 (aa 157–259) | PCR3 | 5A and 6 |
| ps2485 | Fc-EDA1 V365A | Signal-LD-hIgG1 (aa 245–470)-linker-LQVD-hEDA1 (aa 245–391; V365A) | PCR3 | 4 |
| ps2486 | Fc-EDA1 S374A | Signal-LD-hIgG1 (aa 245–470)-linker-LQVD-hEDA1 (aa 245–391; S374R) | PCR3 | 4 |
| ps2487 | Fc-EDA1 Q358E | Signal-LD-hIgG1 (aa 245–470)-linker-LQVD-hEDA1 (aa 245–391; Q358E) | PCR3 | 4 |
| ps2589 | Fc-EDA1 D316G | Signal-LD-hIgG1 (aa 245–470)-linker-LQVD-hEDA1 (aa 245–391; D316G) | PCR3 | 4 |
| ps2590 | Fc-EDA1 T338M | Signal-LD-hIgG1 (aa 245–470)-linker-LQVD-hEDA1 (aa 245–391; T338M) | PCR3 | 4 |
| ps2826 | Fc-hAPRIL | Signal-LD-hIgG1 (aa 245–470)-linker-LQ-hAPRIL (aa 98–233) | PCR3 | 2, B and C |