TABLE 2.

Characteristics of anti-EDA monoclonal antibodies

IGHV and IGLV, immunoglobulin heavy and light chains, respectively, variable region genes likely used in the antibody; Block EDA1, able to prevent the binding of EDA1 to EDAR; Block EDA2, able to prevent the binding of EDA2 to XEDAR; mAb/EDA1 ELISA, number of anti-EDA antibody molecules required to block half of the activity of one trimeric FLAG-EDA1 molecule in the ELISA binding assay; mAb/EDA1 reporter, number of anti-EDA antibody molecules required to block half of the killing activity of one hexameric Fc-EDA1 molecule in the reporter cell assay using EDAR, Fas-expressing Jurkat cells.

Anti-EDA	IGHV gene	IGLV gene	Iso-type	Block EDA1	Block EDA2	mAb/EDA1 ELISA	mAb/EDA1 reporter
EctoD1	1–26	3–4	IgG1	No	No	NA	NA
EctoD2	5–9	3–5	IgG1	Yes	Yes	1.4	4.9
EctoD3	1–81	3–12	IgG1	Yes	Yes	1.1	2.3