FIGURE 5.
Reversible oxidation of mitoNEET [2Fe-2S] clusters by hydrogen peroxide in E. coli cells. E. coli cells containing recombinant human mitoNEET (mito) [2Fe-2S] clusters were resuspended in fresh LB medium to an A600 nm of 4.0. The cells (spectrum 1) were then treated with hydrogen peroxide (1 mm) (spectrum 2), followed by reincubation in LB medium with aeration at 37 °C for 5 min (spectrum 3), 10 min (spectrum 4), and 20 min (spectrum 5). The EPR signal at g = 1.94 indicates the reduced mitoNEET [2Fe-2S] clusters in E. coli cells. The results are representative of three independent experiments. mT, millitesla.