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. 2013 Dec 20;289(7):4316–4325. doi: 10.1074/jbc.M113.522730

FIGURE 3.

FIGURE 3.

pri-155 and mature miR-155 induction are reduced in response to LPS in Ets2−/− mice. A and B, primary peritoneal macrophages from WT, Ets2+/−, or Ets2−/− mice were treated with LPS (100 ng/ml) for 0 or 24 h in vitro. C and D, WT or Ets2−/− mice were administered an intraperitoneal injection of LPS (4 mg/kg) or PBS for 24 h in vivo prior to culling and extraction of peritoneal macrophages. E and F, primary peritoneal macrophages from WT or Ets2−/− mice were treated with LPS (100 ng/ml) for 0 or 24 h in vitro. RT-PCR analysis of RNA was carried out with primers specific for pri-155, miR-155, or SHIP1, as indicated. Expression is normalized to that of GAPDH (pri-155/SHIP1) or RNU6B (miR-155) and is presented relative to that of untreated controls. F, supernatants from peritoneal macrophages stimulated in vitro were harvested, and TNF-α levels were measured by ELISA. In all cases, data are the mean of n = 6–8 mice, with each data point representing an individual mouse, and with error bars representing S.E. Statistical analysis was carried out using Student's t test. *, p < 0.05; **, p < 0.001; ***, p < 0.0001. NT, non-treated.