FIGURE 4.

Self-association of Pilin headgroups. A, log-scale intensity plot of SAXS profiles at 2 mg/ml (green), 5 mg/ml (blue), and 10 mg/ml (magenta). B, superimposition of PilJ x-ray crystal structure (cyan ribbon) into envelope calculated for 2 mg/ml PilJ by SASTBX (gray mesh). C, Kratky Plot (I × q² versus q) at 2 mg/ml (green), 5 mg/ml (blue), and 10 mg/ml (magenta). D, radial distribution function calculated by GNOM at 2 mg/ml (green), 5 mg/ml (blue), and 10 mg/ml (magenta). Error bars are shown as hash marks. The inset shows a Gunier plot (ln(I(q)) versus q²) of the region used to calculate the radius of gyration.