FIGURE 4.
ARV1 knockdown in MIN6 pancreatic β-cells results in decreased triglyceride and increased fatty acid-induced apoptosis. A, mouse ARV1 mRNA expression levels with 0.25 μm ASO treatment for 48 h measured by quantitative RT-PCR. Lipid synthesis measured by [3H]oleate incorporation into TG (B) and PL (C) as a percentage of total lipid species. No significant changes were seen in any other lipid species. Asterisks denote statistical significance (p < 0.05) compared with control ASO, as determined by unpaired t test (mean ± S.D., n = 3). D, quantification of the average number of apoptotic cells based on Hoechst/PI staining after 16 h treatment with indicated concentrations of oleate (18:1), palmitoleate (16:1), or palmitate (16:0) conjugated to fatty acid-free BSA. Asterisks denote statistical significance (p < 0.05) of ARV1-ASO-treated cells (mean ± S.E., 7 fields of 70–200 cells per field) compared with control ASO treatment.