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. Author manuscript; available in PMC: 2014 Jun 12.
Published in final edited form as: Cell Rep. 2013 Dec 5;5(5):1330–1341. doi: 10.1016/j.celrep.2013.11.008

Figure 2. LRP6 is required selectively for excitatory synaptogenesis.

Figure 2

A) (i–iv) Loss of LRP6 leads to reduction in excitatory synapses as evident by reduced number of PSD95 and vGluT1 puncta (n = 20 each). Human LRP6 (hLRP6) that has sequence mismatch with shRNA against mouse/rat LRP6 can rescue the phenotype from effect of shRNA. (v) Loss of LRP6 causes reduction in spine density that can be rescued by hLRP6 (n = 20 each). B) Knockdown of LRP6 does not affect formation of inhibitory synapses, as there was no change in the number of Gephyrin and vGAT puncta in neurons that were transfected with shRNA-LRP6 (n=12). (P < .05, t test). Merged frames show GFP (Green), PSD95 (Red), vGluT1 (Blue) in panel A, and GFP (Green), Gephyrin (Red) and vGAT (Blue) in panel B.