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. Author manuscript; available in PMC: 2014 Jun 12.
Published in final edited form as: Cell Rep. 2013 Dec 5;5(5):1330–1341. doi: 10.1016/j.celrep.2013.11.008

Figure 3. LRP6 is localized at excitatory synapses in mature neurons.

Figure 3

A) Immunolabeling of endogenous LRP6, PSD95 and presynaptic marker vGluT1 reveals that LRP6 is present at excitatory synapses. B) LRP6 does not show any significant level of colocalization with Gephyrin, a postsynaptic marker for inhibitory synapses. Merged frames show LRP6 (Red), PSD95 (Green), and vGluT1/Gephyrin (Blue). C) Quantification of overlap between LRP6 and PSD95 clusters in comparison to LRP6 and Gephyrin clusters reveals significant colocalization between LRP6 and PSD95 but not between LRP6 and Gephyrin. (n= 15 each, P < .001, t test) D) In biochemical fractions of postsynaptic densities from mouse brain endogenous LRP6 is detected in synaptosomal fractions including postsynaptic density fractions 1, 2 and 3 synaptic fractions. Synaptophysin (Synapto.) was used as a presynaptic marker for PSD samples. See also Figure S3.