Figure 2.

The ratio of novel to annotated isoform abundance as determined by reads crossing splice junctions. The number of reads crossing a novel splice junction relative to the corresponding annotated splice junction were used to calculate the ratio. This data demonstrates that a relatively significant portion (~15%) of novel isoforms in the human retinal transcriptome are at least as abundant or more abundant than their annotated counterparts. The green bars represent the percentage of total junctions in which the novel isoform was more abundant than the annotated. The blue-green bar represents the percentage of total junctions where the novel and annotated isoforms are equal (< 2-fold change). The blue bars represent the percentage of total junctions in which the annotated isoforms are more abundant than the novel.