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. 2013 Oct 30;39(4):841–854. doi: 10.1038/npp.2013.284

Figure 5.

Figure 5

Akt phosphorylation and the consequent CREB activation are dependent on the RhoA/ROCK pathway. (a) Representative western blot and densitometric analysis of RhoA in primary hippocampal neurons. RhoA active fraction was expressed as the membrane:cytosol ratio. All the data are expressed as arbitrary units obtained by analyzing the protein bands with Image J software for Windows, for details see the main text. (b) Representative western blot and densitometric analysis of Akt phosphorylation/activation in primary hippocampal neurons. p-Akt protein levels were normalized with total Akt levels and α-tubulin. (c) Representative western blot and densitometric analysis of CREB phosphorylation/activation in primary hippocampal neurons. p-CREB protein levels were normalized with total CREB levels and α-tubulin. All the data are expressed as arbitrary units obtained by evaluating the protein bands with Image J software for Windows, for details see the main text. All the results represent the mean±SD of at least three different experiments. *P<0.05, **P<0.01, and ***P<0.001determined using one-way one-way analysis of variance (ANOVA) followed by the Dunnett post-test. GG, geranylgeraniol; HF, hydroxyfasudil; MVA, mevalonate; Sim, simvastatin; Veh, vehicle.