Figure 3.

Combination of different tumor parameters. (A) The overlay of the vascular (green) and antibody (red) channels showed that the differing density of the vessels leads to an uneven distribution of the antibody. (B) Tumor areas with low vascular density showed only very slight or absent antibody signals, whereas (C) in areas with a high vascular density, there was strong and homogeneous antibody penetration. (D) The combination of the tissue (gray) and vascular information clearly showed the correlation between vascular density and the development of necrosis. (E) Areas with low or absent vascular connections developed necrosis, whereas (F) those with a high vascular density had a solid tissue structure. In the latter, erythrocytes (white spots) were also more prevalent. (G) The overlapping of the tissue and antibody channel showed the penetration of the trastuzumab-Alexa 750 in the different areas of tissue. (H) Necrotic areas of the tumor were hardly penetrated by the labeled antibody at all. (I) Solid tumor tissue, however, demonstrated very strong and homogeneous antibody penetration and distribution. (A–I) MIP, 30 slices with 5-µm diameter per slice. Scale bar, 250 µm and 100 µm (blowup).