(A). Growth curves of recipient bacteria with genes encoding the B.
ovatus inulin PLs (BACOVA_04502 and 04503), the B.
thetaiotaomicron levan GH (BT_1760), the B.
thetaiotaomicron amylopectin GH (susG, BT_3698),
or vector alone (pFD340) in defined polysaccharide media. The initial decrease
in OD600 in amylopectin and levan media correspond to rapid
degradation of these optically dense polysaccharides. (B) TLC analyses of PBP
released from recipient strains containing GH/PL genes in
trans, or vector alone (pFD340) through the growth phases (inulin and
amylopectin: 1-early, 2-mid, 3-late log, -4 stationary; levan: 1-lag, 2-early,
3-mid, 4-late log, 5-stationary). As recipient with vector alone did not grow in
polysaccharide media, its time points correspond to the growth points of
recipient strains containing GH/PL genes in trans. Data are
representative of ≥3 independent experiments, one representative
experiment is shown.