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. Author manuscript; available in PMC: 2015 Jan 6.
Published in final edited form as: Curr Biol. 2013 Dec 12;24(1):40–49. doi: 10.1016/j.cub.2013.10.077

Figure 3. Primary growth and PBP liberation by recipients containing producer- derived GH/PL genes.

Figure 3

(A). Growth curves of recipient bacteria with genes encoding the B. ovatus inulin PLs (BACOVA_04502 and 04503), the B. thetaiotaomicron levan GH (BT_1760), the B. thetaiotaomicron amylopectin GH (susG, BT_3698), or vector alone (pFD340) in defined polysaccharide media. The initial decrease in OD600 in amylopectin and levan media correspond to rapid degradation of these optically dense polysaccharides. (B) TLC analyses of PBP released from recipient strains containing GH/PL genes in trans, or vector alone (pFD340) through the growth phases (inulin and amylopectin: 1-early, 2-mid, 3-late log, -4 stationary; levan: 1-lag, 2-early, 3-mid, 4-late log, 5-stationary). As recipient with vector alone did not grow in polysaccharide media, its time points correspond to the growth points of recipient strains containing GH/PL genes in trans. Data are representative of ≥3 independent experiments, one representative experiment is shown.