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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: DNA Repair (Amst). 2013 Jul 18;12(9):774–785. doi: 10.1016/j.dnarep.2013.06.004

Fig. 3.

Fig. 3

Characterization of XRCC1 NTDs. (A) CD melting curves (at 225 nm) for reduced wild-type XRCC1 NTD and C12A NTD. The Tm was 54.3 °C for wild-type (closed symbols) and 50.6 °C for C12A (open symbols). Studies were performed on 5 μM protein in 20 mM Tris, pH 7.35, 140 mM NaCl, 5 mM TCEP. (B) Determination of pol β catalytic domain/XRCC1 NTD binding affinities. Titration of aminosulfonylbenzofuran (ABD)-labeled pol β P300C mutant with reduced wild-type XRCC1 NTD (closed symbols) and mutant C12A XRCC1 NTD (open symbols). Curves are fit to a two-state binding isotherm.