Table 6.
Primer sequences used for validation in qRT-PCR analysis
| Name | Blast SOL network genomics | Annotation | Foward sequence | Reverse sequence |
|---|---|---|---|---|
| 0U17 |
NM |
Protein |
ACTACCAACATTCACAGCAGCTC |
TTAACCCTGTTGAAGGTTAGTGC |
| 0U6 |
SGN E1316291 |
Acid phosphatase |
CTAATTAACCCTCTCCGCATGAT |
GCCAACTCAGGCAATTATATACG |
| 0U14 |
SGN E667484 |
Protein |
TAGTCAAGAATATGGGCATGGAC |
ATACCTTCTTGATTCACGCCTTC |
| 0U2 |
SGN E1352064 |
Caffeine synthase |
AAAGGGAGCATTTACTCTTCCAAAG |
AGCATGCATCCTGAGAAATGTGGTA |
| 0U4 |
SGN E1310344 |
Metallothionein-like protein |
ATTCGTCTGCTCTGTGAAGATGT |
ATACATGTTTCCGCAGTTTCCT |
| 0U322 |
SGN E450221 |
nadp-dependent d-sorbitol-6-phosphate dehydrogenase |
CCTTTGTGGCTTCTAAGCAAAT |
GGAAAGCAGAGATTGACAAACAG |
| 0U1 |
SGN E628893 |
No hits |
CAAGGAAGATGCTTTTGACGAT |
TGTAATTATGCTGCTGGTGCTAC |
| 0U1 |
SGN E628893 |
No hits |
CATTTAGTTTGGAAGGGGACAA |
GGATACAGCCGGTAGGACTAACT |
| 0U42 |
SGN E636199 |
---NA--- |
ACCCGCCGGGAAACC |
GATGCACAGACAGGAATCACAAC |
| 0D6 |
SGN E661762 |
---NA--- |
TTGGTAATGATGGAAGTGTCCTC |
GCCAGTAATGGGATTGTAGTTGA |
| 0D10 |
SGN E1033676 |
Glycerol-phosphate acyltransferase |
CTAGCTTGACCAGGAAAGACAAG |
GACTCAGGACTGCTCATTTCATT |
| 0D2 |
SGN E676870 |
Protein |
GGCAACTACTGCATTCTATCAGC |
AAATGGATGAGCTGAAGGAGAAC |
| 0D1 |
SGN E1320843 |
Protein |
ACTAGTACTGGGTGTTGCCTCAA |
GGTGAGCAAATAGTTGTTGTTGC |
| 0D19 |
SGN E673783 |
Isocitrate lyase |
GGCCAGGAGCAACAGACATT |
ATTCTCTCACAATCTTGACTTTGCA |
| 0D3 |
SGN E1320843 |
Zinc finger |
CTGATTACGTCCGCTATCTCATT |
AACCTATCGGACCTGTACCTGTT |
| 0D4 |
SGN E1325444 |
Hypothetical protein |
TACTGGCACTAATGGAGGAAATG |
AATGGGGACAGATGTATCATCAC |
| 0D8 |
NM |
Glycerol-3-phosphate acyltransferase 6 |
TTCAAGAGTTTGGTACTGACGTG |
ATCATGGTCTGTCTCTCGATCTC |
| 0D9 |
SGN E1321887 |
gdsl-motif lipase hydrolase-like |
TCTTAACTGGACTTCCTCCAATG |
CCATTGAAGTTTAGAGCCACAAC |
| 0D7 |
NM |
Hypothetical protein |
CCAAGACAGTTGATCTCCCTCTA |
CGTAGTAGCTAGATGGTGCCAGT |
| 0U9 |
SGN-E1337775 |
Organ-specific protein |
GGTTTCTTTAGGGTTTCCTTCCT |
CACAGTGTGTGTGTTTTGTTCCT |
| 0U11 |
SGN-E1352070 |
Caffeine synthase |
CCTAGCAAGCCATTTTGGAG |
ATTCTTGGCAAACCTGTGGA |
| 0U6 |
SGN-E1316291 |
Acid phosphatase |
ATTACGGCTATGGCAGAATTAGC |
CACCATGTTTCCTTGTTTGAGA |
| 0U3 |
SGN-E1326397 |
Acidic endochitinase se2 |
CAGCAAATTCTTCCCTATGTCC |
CAGCGTTTCAGGGTTAACATAAG |
| 0U8 | SGN-E1327615 | Kunitz trypsin inhibitor | CTCTTCCTTTCATTTCTGCTCTTC | GACGTAGTACTCGACACCAGGAC |