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Hemolytic activities of NP1-NP9 (500 nM each) in the absence of plasma proteins. RBCs were incubated with NPs for 30 min in PBS at 37 °C and the mixture was centrifuged to detect the cell-free hemoglobin in the supernatant. % Hemolysis was calculated using water as the positive control. Error bars represent standard deviation (n=3).
